Exploring the feasibility of solvent recirculation in the extraction process of (poly)phenols from elderflower (Sambucus nigra L.)

Nebojša Vasiljević; Vladan Mićić; Zoran Petrović; Duško  Kostić; Nikolina  Palangetić; Jelena  Vuković

doi:10.5937/ror2401037V

Autori

Nebojša Vasiljević University of East Sarajevo, Faculty of Technology, Zvornik, Republic of Srpska, B&H Author https://orcid.org/0000-0002-3789-9975
Vladan Mićić University of East Sarajevo, Faculty of Technology, Zvornik, Republic of Srpska, B&H Author https://orcid.org/0000-0002-4383-4879
Zoran Petrović University of East Sarajevo, Faculty of Technology, Zvornik, Republic of Srpska, B&H Author
Duško Kostić University of East Sarajevo, Faculty of Technology, Zvornik, Republic of Srpska, B&H Author https://orcid.org/0000-0002-4115-8571
Nikolina Palangetić University of East Sarajevo, Faculty of Technology, Zvornik, Republic of Srpska, B&H Author
Jelena Vuković University of East Sarajevo, Faculty of Technology, Zvornik, Republic of Srpska, B&H Author

DOI:

https://doi.org/10.5937/ror2401037V

Ključne reči:

antocijani, cvet zove, ekstrakcija, flavonoidi, (poli)fenoli

Apstrakt

U ovom radu izvedena je dvostepena ekstrakcija čvrsto - tečno tradicionalnom tehnikom maceracije. Nakon prvog koraka ekstrakcije, ekstrakt je odvojeno istrošenog biljnog materijala (cvet zove), nakon čega je sveži cvet zove dodat ekstraktu. Eksperimentalni parametri uključivali su različite koncentracije etanola (30 %, 60 % i 90 %), odnose čvrsto-tečno (1:15, 1:30 i 1:45 m/v), pH vrednosti (3, 7 i 10) i vremena ekstrakcije (15, 30 i 45 minuta), dok je jedan faktor u vremenu (One-factor-at-a-time (OFAT)) korišćen kao eksperimentalni dizajn. Rezultati su pokazali da je optimalna koncentracija etanola u rastvaraču 60 vol.% za oba koraka ekstrakcije. Povećanje odnosa čvrsto-tečno sa 1:15 m/v na 1:30 m/v rezultuje većim sadržajem (poli)fenola, dok je blagi pad sadržaja (poli)fenola primećen kod većih odnosa čvrsto-tečno. Sadržaj (poli)fenola je približno isti u kiseloj i neutralnoj sredini, dok je u baznoj sredini manji u odnosu na kiselu i neutralnu sredinu. Iako sadržaj (poli)fenolnih jedinjenja raste sa produženjem vremena ekstrakcije, 30 minuta je optimalno vreme budući da se daljim produženjem ne povećava značajno njihov sadržaj. Dodatno, primećeno je da je ukupan sadržaj (poli)fenola bio niži u drugom koraku ekstrakcije, što sugeriše na zasićenje rastvarača nakon prvog ekstrakcionog koraka.

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