Selective bio-flocculation of hematite mineral using Bacillus subtilis for phosphorous removal from iron ores

Samah S. Abdallah; Samah M. El-Sayed; Mohamed A. Abdel-Khalek

doi:10.5937/ror2501067A

Authors

Samah S. Abdallah Central Metallurgical Research and Development Institute (CMRDI), Cairo, Egypt Author https://orcid.org/0000-0002-5824-7941
Samah M. El-Sayed Central Metallurgical Research and Development Institute (CMRDI), Cairo, Egypt Author
Mohamed A. Abdel-Khalek Central Metallurgical Research and Development Institute (CMRDI), Cairo, Egypt Author

DOI:

https://doi.org/10.5937/ror2501067A

Keywords:

Bacillus Subtilis, phosphorous, Hematite, apatite, bio-flocculation, minerals

Abstract

Bacillus subtilis is used as a bio-surface modifier to flocculate hematite mineral (Fe 2O 3). The effect of bacterial interaction on the behavior of hematite and apatite minerals, as the main source of phosphorous in iron ore, was investigated using zeta-potential, SEM, and FTIR measurements. The effects of pH, contact time, and Bacillus subtilis concentration on the flocculation of the two minerals were studied. The zeta potential of hematite is strongly affected by interaction with Bacillus subtilis, and maximum flocculation efficiency was achieved at pH 6. Hematite could be separated from its mixture with apatite in the presence of 8 - 12×10 5 cells of Bacillus subtilis at pH 6 and 35 °C for a 10-minute contact time.

References

Abdallah S. S., Abdel-Khalek N. A., Farghaly M. G., Selim K. A., Abdel-Khalek M. A., Role of Pseudomonas songnenensis-Apatite Interaction on Bio-Flotation of Calcareous Phosphate Ore, Biointerface Research in Applied Chemistry, 11 (6), 2021, 14451-14462

https://doi.org/10.33263/BRIAC116.1445114462

Abdallah S. S., El Sayed S. M., Abdel-Khalek M.A, Selective bio-flocculation of hematite mineral using Bacillus subtilis for phosphorous removal from iron ores, Proceedings - XVI International Mineral Processing and Recycling Conference, IMPRC, 28-30 May 2025, Belgrade, Serbia, 313-319

https://doi.org/10.5937/IMPRC25313A

Abdel-Khalek M. A., El-Maidany A. A., Application of Bacillus subtilis for reducing ash and sulfur in coal, Environmental Earth Sciences, 70, 2013, 753-760

https://doi.org/10.1007/s12665-012-2163-4

Abdel-Khalek M. A., Elmaidany A. A., Coal-Mycobacterium phlei interaction and its effect on coal cleaning, Tenside Surfactants Detergents, 50 (6), 2013a, 414-419

https://doi.org/10.3139/113.110273

Abdel-Khalek M. A., Elmaidany A. A, Effect of Paenibacillus polymyxa on surface properties and cleaning of coal, Fuel Processing Technology, 113, 2013b, 52-56

https://doi.org/10.1016/j.fuproc.2013.03.016

Abdel-Khalek N. A., El-Shall H., Abdel-Khalek M. A., El-Mahdy A. M., El-Mofty S. E., Carbonate separation from sedimentary phosphates through bioflotation, Mining, Metallurgy & Exploration, 26, 2009, 85-93

https://doi.org/10.1007/BF03403423

Ahmad M., Ahmad I., Hilger T. H., Nadeem S. M., Akhtar M. F., Jamil M., Hussain A., Zahir Z. A., Preliminary study on phosphate solubilizing Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 for improving cotton growth under alkaline conditions, PeerJ. 4:6:e5122, 2018

https://doi.org/10.7717/peerj.5122

Ahmad I., Ahmad M., Hussain A., Jamil M., Integrated use of phosphate-solubilizing Bacillus subtilis strain IA6 and zinc-solubilizing Bacillus sp. strain IA16: a promising approach for improving cotton growth, Folia Microbiologica, 6, 2021, 115-125

https://doi.org/10.1007/s12223-020-00831-3

Alangari A., Alqahtani M., Mateen A., Abul-Kalam M., Alshememry A., Ali R., Kazi M., AlGhamdi K., Syed R., Iron Oxide Nanoparticles: Preparation, Characterization, and Assessment of Antimicrobial and Anticancer Activit, Adsorption Science & Technology, 2022, 1-9, 1562051

https://doi.org/10.1155/2022/1562051

Bao Q., Guo L., Guo Z., A novel direct reduction-flash smelting separation process of treating high phosphorous iron ore fines, Powder Technology, 377, 2021,149-162

https://doi.org/10.1016/j.powtec.2020.08.066

Benbow, J., Bridgwater J., Paste Flew and Extrusion, 1st Edition, Oxford University Press, Inc, New York, 1993, 176

Buis P., Bioremediation techniques for the removal of phosphorus from iron ore, (Dissertation - PhD for Mining Engineering), Michigan: Michigan Technological University, USA, 1995, 129

Elmahdy A. M., El-Mofty S. E., Abdel-Khalek M. A., Abdel-Khalek N. A., El-Midany A. A., Bacterially induced phosphate-dolomite separation using amphoteric collector, Separation and Purification Technology, 102, 2013, 94-102

https://doi.org/10.1016/j.seppur.2012.09.033

Elmahdy A. M., El-Mofty, S. E., Abdel-Khalek M. A., Abdel-Khalek N. A., El-Midany A. A., Dolomite-apatite separation by amphotericcollector in presence of bacteria, Journal of Central South University of Technology, 20, 2013a, 1645-1652

El-Ghammaz M. R., Abdel-Khalek N. A., Hassan M. K., Proteomic profile to explain the mechanism of the Bacillus cereus-phosphate mineral interaction, Physicochemical Problems of Mineral Processing, 57 (3), 2021, 136-150

https://doi.org/10.37190/ppmp/136312

El-Sayed S., El-Shatoury E. H., Abdel-Khalek N. A., Abdel-Motelib A., Abdel-Khalek M. A., Influence of Bacillus cereus-Gold Interaction on Bio-flotation of Gold in the Presence of Potassium Butyl Xanthate, Biointerface Research in Applied Chemistry, 11 (5), 2021, 13005-13018

https://doi.org/10.33263/BRIAC115.1300513018

Farghaly M. G., Abdel-Khalek N. A., Abdel-Khalek M. A., Selim K. A., Abdullah S. S., Physicochemical study and application for pyrolusite separation from high manganese-iron ore in the presence of microorganisms, Physicochemical Problems of Mineral Processing, 57 (1), 2021, 273-283

https://doi.org/10.37190/ppmp/131944

Feng B., Lu Y., Feng Q., Zhang M., Gu Y., Talc-serpentine interactions and implications for talc depression, Minerals Engineering, 32, 2012, 68-73

https://doi.org/10.1016/j.mineng.2012.03.004

Geoghegan M., Andrews J., Biggs C., Eboigbodin K., Rolfe S., Scholes J., Ojeda J. J., The Polymer Physics and Chemistry of Microbial Cell Attachment and Adhesion, Faraday Discussions, 139, 2008, 85-103

https://doi.org/10.1039/b717046g

Halcomb D. W., Young R. A., Thermal decomposition of human tooth enamel, Calcified Tissue International, 31, 1980, 189-201

https://doi.org/10.1007/BF02407181

Hori K., Matsumoto S., Bacterial adhesion: From mechanism to control, Biochemical Engineering Journal, 48 (3), 2010, 424-434

https://doi.org/10.1016/j.bej.2009.11.014

Hussein F. B., Venkiteshwaran K., Mayer B. K., Cell surface-expression of the phosphate-binding protein PstS: System development, characterization, and evaluation for phosphorus removal and recovery, Journal of Environmental Sciences, 92, 2020, 129-140

https://doi.org/10.1016/j.jes.2020.02.016

Hwang S., Umar A., Dar G. N., Kim S., Badran R., Synthesis and Characterization of Iron Oxide Nanoparticles for Phenyl Hydrazine Sensor Applications, Sensor Letters, 12 (1), 2014, 97-101(5)

https://doi.org/10.1166/sl.2014.3224

Ishizawa H., Kuroda M., Inoue D., Ike M., Genome-wide identification of bacterial colonization and fitness determinants on the floating macrophyte, duckweed, Communications Biology, 5(1):68, 2002

https://doi.org/10.1038/s42003-022-03014-7

Kandori K., Horigami N., Yasukawa A., Ishikawa T., Texture and Formation Mechanism of Fibrous Calcium Hydroxyapatite Particles Prepared by Decomposition of Calcium-EDTA Chelates, Journal of the American Ceramic Society, 80 (5), 1997, 1157-1164

https://doi.org/10.1111/j.1151-2916.1997.tb02958.x

Kosmulski M., The pH dependent surface charging and points of zero charge. VIII. Update, Advances in Colloid and Interface Science, 275, 2020, 102064

https://doi.org/10.1016/j.cis.2019.102064

Kuyumcu H. Z., Vilinska T. B., Rao K. H., Biocoagulation and its Application Potentials for Mineral Bioprocessing, The Open Mineral Processing Journal, 2, 2009, 1-11

https://doi.org/10.2174/1874841400902010001

Laible A., Dinius A., Schrader M., Krull R., Kwade A., Briesen H., Schmideder S., Effects and interactions of metal oxides in microparticle-enhanced cultivation of filamentous microorganisms, Engineering in Life Sciences, 22, 2021, 725-743

https://doi.org/10.1002/elsc.202100075

Li Q., Dong F., Dai Q., Zhang C., Yu L., Surface properties of PM2.5 calcite fine particulate matter in the presence of same size bacterial cells and exocellular polymeric substances (EPS) of Bacillus mucitaginosus, Environmental Science and Pollution Research, 25 (23), 2018, 22429-22436

https://doi.org/10.1007/s11356-017-0829-x

Mohamed E., Farag A., Youssef S., Phosphate Solubilization by Bacillus subtilis and Serratia marcescens Isolated from Tomato Plant Rhizosphere, Journal of Environmental Protection, 9 (3), 2018, 266-277

https://doi.org/10.4236/jep.2018.93018

Natarajan K. A., Deo N., Role of bacterial interaction and bioreagents in iron ore flotation, International Journal of Mineral Processing, 62(1-4), 2001, 143-157

https://doi.org/10.1016/S0301-7516(00)00049-1

Nunes A. P. L., Pinto C. L. L., Valadão G. E. S., de Magalhães Viana P. R., Floatability studies of wavellite and preliminary results on phosphorus removal from a Brazilian iron ore by froth flotation, Minerals Engineering, 39, 2012, 206-212

https://doi.org/10.1016/j.mineng.2012.06.004

Ofoegbu S. U., Technological Challenges of Phosphorus Removal in High-Phosphorus Ores: Sustainability Implications and Possibilities for Greener Ore Processing, Sustainability, 11, 2019, 6787

https://doi.org/10.3390/su11236787

Ramírez-Aldaba H., Vazquez-Arenas J., Sosa-Rodríguez F. S., Valdez-Pérez D., Ruiz-Baca E., García-Meza J. V., Trejo-Córdova G., Lara R. H., Assessment of biofilm changes and concentration-depth profiles during arsenopyrite oxidation by Acidithiobacillus thiooxidans, Environmental Science and Pollution Research, 24 (24), 2017, 20082-20092

https://doi.org/10.1007/s11356-017-9619-8

Rao K. H., Vilinska A., Chernyshova I. V., Minerals bioprocessing R & D needs in mineral bio-beneficiation, Hydrometallurgy, 20 (3-4), 2010, 465-470

https://doi.org/10.1016/j.hydromet.2010.01.016

Rong X. M., Huang Q. Y., He X. M., Chen H., Cai P., Liang W, Interaction of Pseudomonas putida with kaolinite and montmorillonite: A combination study by equilibrium adsorption, ITC, SEM and FTIR. Colloids and Surfaces B: Biointerfaces, 64 (1), 2008, 49-55

https://doi.org/10.1016/j.colsurfb.2008.01.008

Soejoko D. S., Tjia M. O., Infrared spectroscopy and X-ray diffraction study on the morphological variations of carbonate and phosphate compounds in giant prawn (Macrobrachiumrosenbergii) skeletons during its moulting period, Journal of Materials Science, 38, 2003, 2087-2093

https://doi.org/10.1023/A:1023566227836

Shashikala A. R., Raichur A. M., Role of interfacial phenomena in determining adsorption of Bacillus polymyxa onto hematite and quartz, Colloid and Surfaces B: Biointerfaces, 24 (1), 2002, 11-20

https://doi.org/10.1016/S0927-7765(01)00217-X

Tsezos M., Biological Removal of Ions: Principles and Applications, Advanced Materials Research, 20-21, 2007, 589-596

https://doi.org/10.4028/www.scientific.net/AMR.20-21.589

van Oss C. J., Review of Microbial Cell Surface Analysis: Structural and Physicochemical Methods, Mozes, N.; Handley, P.S.; Busscher, H.J.; Rouxhet, F.G. (eds.), VCH Publishers, New York, 1991, Journal of Dispersion Science and Technology, 13 (1), 1992, 115-117

https://doi.org/10.1080/01932699208943299

Wang S., Liu L., Li H., Fang F., Yan P., Chen Y., Guo J., Ma T., Shen Y., The branched chains and branching degree of exopolysaccharides affecting the stability of anammox granular sludge, Water Research, 178, 2020, 115818

https://doi.org/10.1016/j.watres.2020.115818

Wen-tang XIA, Zheng-de REN, Yi-feng GAO, Removal of Phosphorus from High Phosphorus Iron Ores by Selective HCl Leaching Method, Journal of Iron and Steel Research, International, 18 (5), 2011, 1-4

https://doi.org/10.1016/S1006-706X(11)60055-1

Wu S. C., Li Z. Y., Sun T. C., Kou J., Li X. H., Effect of additives on iron recovery and dephosphorization by reduction roasting-magnetic separation of refractory high-phosphorus iron ore, International Journal of Minerals, Metallurgy and Materials, 28, 2021, 1908-1916

https://doi.org/10.1007/s12613-021-2329-8

Xu C. Y., Sun T. C., Kou J., Li Y. L., Mo X. L., Ligang T., Mechanism of phosphorus removal in beneficiation of high phosphorous oolitic hematite by direct reduction roasting with dephosphorization agent, Transactions of Nonferrous Metals Society of China, 22 (11), 2012, 2806-2812

https://doi.org/10.1016/S1003-6326(11)61536-7

Zhu D. Q., Chun T. J., Pan J., Lu L. M., He Z., Upgrading and dephosphorization of Western Australian iron ore using reduction roasting by adding sodium carbonate, International Journal of Minerals, Metallurgy, and Materials, 20 (6), 2013, 505-513

https://doi.org/10.1007/s12613-013-0758-8